QI® Cryo – the Science of Cool

Linde cryogenic solutions provide the next generation of freezers for reliable, energy-efficient and cost-effective freezing.

Efficiency and safety using cryogenic technology

The use of cryopreservation technology in preserving biological materials has revolutionized the field of medical research. With the advent of liquid nitrogen freezers, the process of cryopreservation has become more efficient, reliable and cost-effective. Linde offers innovative and efficient liquid and vapor phase nitrogen freezers, designed for a variety of storage temperatures from -20 to -190°C. Our advanced vacuum technology sets us apart from the competition. Built with cutting-edge engineering, our equipment maintains consistent temperatures for optimal storage of biological materials. We take pride in offering the widest range of products on the market suitable for storage of biological materials. From liquid nitrogen and vapor phase freezers to Dewars and storing accessories, Linde has everything you need for efficient cryopreservation. Our broad expertise allows us to design and install "turn-key" solutions systems that are efficient, economical in their use of liquid nitrogen, and provide the best possible return of your storage investment.

Advantages of cryopreservation with liquid nitrogen compared with mechanical freezers

Cryopreservation is the process of preserving biological materials at extremely low temperatures. While mechanical freezers have been traditionally used in this process, liquid nitrogen freezers offer several advantages that make them more efficient and economical than mechanical freezers.

Liquid nitrogen freezers are reliable and bringing huge savings on maintenance costs.

Choosing a liquid nitrogen freezer provides an environment free of noise and heat created by mechanical freezer.

Lower temperatures achieved by liquid nitrogen freezers provide greater safety and a longer life for the samples.

Every freezer is designed with optimum vacuum performance.

Freezer structure enables sample to be stored in vapor or in liquid phase.

With these advantages, researchers can be confident that their biological materials are preserved optimally for long-term storage and for the future use.

Did you know?

Nitrogen is an inert gas, it is stable – there are no hazardous reactions with other substances.

Liquid nitrogen (LIN) is a colorless, odorless and tasteless. LIN is extremely cold, with a boiling point of -196°C (-320°F).

Safety measures must be taken when handling liquid nitrogen, as it may cause cryogenic burns or injury! Wear always cold insulating gloves and face shield.

Risk of suffocation – oxygen deficiency. Oxygen monitoring alarm system is needed in areas where liquid nitrogen is used.

Automated liquid nitrogen freezers

Linde represents the world leading manufacturer of vacuum insulated products, MVE Biological Solutions. HeCo Series Freezers -190 °C are available in three unique sizes and provide maximum storage density from 15,600 to 94,500 biological sample vials. They are one of the most efficient vapor freezers on the market. Features include:

Dry sample storage

-190 °C top box temperature

Efficient usage of liquid nitrogen

The security holding period is approximately 2 weeks before the temperature rises from -190 °C to -130 °C after the latest filling

Automated monitoring system with liquid level control, temperature alarm and log

Individual freezers can be equipped with a 230-litre storage tank, with automatic feeding of liquid nitrogen. For larger cryopreservation systems, liquid nitrogen is fed through vacuum-isolated pipelines from an external tank to the freezers.

NEW innovative and energy efficient alternative for ultra-low temperature to cryogenic storage

Linde together with MVE Biological Solutions offers the completely dry sample storage freezer. It will maintain a user-defined temperature anywhere between -20°C and -150°C.

The MVE Variõ® Series significantly reduces the possibility of sample contamination via contact with LN2 while providing the safety margin and consistent temperature profile, even with the lid open, that is associated with LN2 based cryogenic storage.

The MVE Variõ Series is able to provide all of this with less than 1% of the power consumption and approximately 70% overall operating cost savings when compared to the leading mechanical freezers.

MVE Variõ® Series Freezer

What do customers say?

“Another thing that is really important to us is sample safety. So in case of lack of electricity we can keep the samples frozen for two weeks after the last liquid nitrogen refill, which wouldn’t be possible with electrical freezers, in which you typically have to evacuate the samples within 24 hours.”

Tiina Vesterinen,
FIMM Sample Storage Helsinki Laboratory Coordinator
HiLIFE, University of Helsinki

Downloads

Safety datasheets Product datasheets & brochures

Would you like to learn more or ask for advice about cryogenic preservation?